Tutorials: Learn How to Use DBTMEE

Question: Which TFs are the potential regulators for fertilization-specific genes? What about the expression patterns of such TFs (Is it a sperm-derived factor?)

The Regulation Browser provides information on the inference of TF-Gene interactions for specific gene subsets that are not expressed at oocytes. Although users can enter an asterisk (*, a wildcard that matches all gene names), we have entered "Foxd1" into the Gene field (Step 2) in this tutorial. Then, users must select "Oo_absent_genes" from the Table selection field. This table includes genes that have been categorized into six groups:

In the case of "Foxd1", the result shows that 62 TFs are putatively binding to the promoter of "Foxd1" that is a member of "Nsc1C" category (i.e. a fertilization specific gene, see CASE 1) (Step 3). By clicking the number in the Bound-TFs column, uses can see the TF-Gene connection and the list of 62 TFs (Step 4). The result suggests that Foxd1 binds to the promoter of itself. The list includes hyperlinks to the information of genes (Step 5).