Tutorials: Learn How to Use DBTMEE

It has been reported that the factors enriched in oocytes and zygotes are good candidates to enhance the reprogramming of somatic cells to iPSCs; e.g. Glis1 (Maekawa et al. PUBMED), Hist1h2aa (TH2A), Hist1h2ba (TH2B) (Shinagawa et al. PUBMED). The characteristics of these factors are high gene expression levels at oocytes and zygotes (Maternal RNA category). In contrast, they exhibit low gene expression levels at ESCs and iPSCs [Step0-1, 0-2]. Moreover, though these TFs are the genes which are not expressed in sperm, they are significantly enriched by H3K4me3 (an active histone mark) in sperm [Step0-3, 0-4, 0-5]. H1foo exhibits the similar expression pattern, but H1foo is marked by neither histones in sperm. This linker histone does not enhance the generation of iPSCs (Shinagawa et al, PUBMED).

To identify such potential factors for the reprogramming, we set up thresholds to the Advanced Search option in Table Browser [Step 1];